AFAB

Keywords: amino acids, cyclosporin A, Fermentation, production, response surface method, static, stationary culture, Tolypocladium inflatum

Abstract:

The effect of different nutrients (carbon and nitrogen sources) and fermentation factors (such as inocu- lum size and volume of production media) was evaluated on the production of Cyclosporin A (CyA) in sta- tionary culture of Tolypocladium inflatum MTCC 557 over a 21-day period at 25oC. Glycerol was found to be the best carbon source and gave a maximum CyA production of 410 mg/L. Further, response surface meth- odology was used to optimize the concentrations of medium components which took the CyA production to 452 mg/L. The exogenous supplementation of various amino acids individually and in combination was also studied. The time of addition of the optimized combination of amino acids was also evaluated. A maximum CyA production of 1241 mg/L was obtained when L-valine and L-leucine were added after 4th day of the fermentation. The optimum concentrations of media components were (in g/L) glycerol 98.8, casein peptone 28.5, malt extract 20, peptone 10 and α-amino butyric acid 5.7.

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