Isolation and Initial Characterization of Plasmids in an Acetogenic Ruminal Isolate
Published 11/2011
Volume 1 Issue 2
Pp. 186-192
Keywords: acetogens, bacteria, characterization, genetics, Isolation, plasmids, rumen
Abstract:
Two of nine acetogenic ruminal isolates screened for plasmids were found to contain plasmid DNA. Five plasmids ranging in size from 4.5 to 32 kilobase pairs (kb) were observed in isolate H3HH while a single 35 kb plasmid was observed in isolate H4. The smallest of the plasmids from isolate H3HH, estimated at 4.5 kb, was isolated using gel electrophoresis followed by electroelution. Of the 13 restriction endonucleases tested, this plasmid was cut once by EcoRV, SinI and HindIII and cut twice BglII. The physiological functions of the individual plasmids are unknown. However, a plasmid-free derivative (H3HP) of isolate H3HH displayed increased sensitivity to several antibiotics.
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